L07 Chem 550 Mass Spectrometry

This course covers the fundamentals of instrumentation, ionization, and gas-phase ion chemistry. Magnet sector, quadrupole, time-of-flight, ion trap, and Fourier transform instruments are considered. Ionization methods include the venerable electron ionization, chemical ionization, and fast atom bombardment, plus the newer electrospray and matrix-assisted laser desorption methods. Mechanisms of gas-phase ion decomposition reactions, rates and thermodynamics of gas-phase ion processes, and ion-molecule reactions are discussed particularly in terms of their intrinsic interest and for interpreting spectra. Combined or hyphenated methods such as GC/MS, LC/MS, and tandem mass spectrometry are also discussed. A second major focus includes applications in a variety of areas: structure determination of synthetic, natural-product, and biomolecules, exact mass measurements (high resolution MS, peptide and protein sequencing, trace analysis, sensitive detection, and mixture analysis. Prerequisite: Chem 252 or permission of instructor.

Credit 3 units.

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